Real Time PCR Reagents and Kits
Products designed for use during real-time (quantitative) PCR procedures. Includes master mixes, DNA polymerases, reverse transcriptases, size standards, kits, and reagents.
Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique that combines the reverse transcription of RNA into DNA and the subsequent amplification of the DNA using PCR.
In RT-PCR, a reverse transcriptase enzyme converts an RNA template to its complementary DNA (cDNA). The cDNA then becomes the template for PCR amplification.
A typical RT-PCR kit includes the main components needed for the assay:
- Buffer
- Primers – a combination of deoxynucleotide triphosphates (dNTPs) of the four bases of adenine (dATP), cytosine (dCTP), guanine (dGTP), and thymine (dTTP)
- Reverse transcriptase reagent
The combination of RT-PCR and qPCR techniques (sometimes labelled RT-qPCR) is often used in research and clinical labs to analyze gene expression and quantify viral RNA. Since this method uses fluorescence to track the amplification reaction, these kits may also include:
- Fluorogenic primers or labeled fluorogenic probes
- Fluorescent dye
Reagents for cDNA PCR can be purchased separately or as cDNA synthesis kits.
RT-PCR is commonly used for RNA detection, molecular cloning, and gene sequencing. RT-qPCR is used for gene expression analysis, RNAi or microarray validation, pathogen detection, genetic testing, and other disease research.
Integrated DNA Technologies Inc INTEGRATED DNA TECHNOLOGIES INC
NC4034426 PRIMETIME- XL QPCR ASSAY TBP
Andwin Scientific QSCRIPT 1STEP TOUGHMX 500X20UL
The qScript XLT One-Step RT-qPCR ToughMix by Quanta Biosciences is a ready-to-use, highly sensitive master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates with the use of hybridization probe detection chemistries like the TaqMan 5-hydrolysis probes on real-time PCR systems that do not need a passive reference dye. This product contains: reagent description, qScript XLT One-Step RT-qPCR ToughMix (2X), 2X reaction buffer containing dATP, dCTP, dGTP, dTTP, magnesium chloride, qScript XLT reverse transcriptase, RNase inhibitor protein, hot-start DNA polymerase, AccuVue blue qPCR dye, and stabilizers.
Sigma Aldrich Fine Chemicals Biosciences LuminoCtR qPCR ReadyMixTM
LuminoCt(R) qPCR ReadyMix combines the performance enhancements of our JumpStart(TM) Taq antibody for hot start PCR with the convenience of an easy-to-use reaction mixture. This is the ideal SOLUTIONon for performing high-throughput quantitative PCR methods that rely on a fluorescent probe.
Alkali Scientific Radiant™ Probe 1-Step Lo-ROX qPCR Kit, 300 Reactions, (3 X 1mL Probe 1-Step Lo-ROX 2X qPCR Mix, 3 x 0.2mL 20X RTase with RNase Inhibitor)
Alkali Scientific offers Radiant™ Probe 1-Step Lo-ROX qPCR Kits, a high-performance portfolio of 2X ready-to-use real-time PCR reagents designed for best-in- class quantitative PCR. Radiant™ qPCR Kits are engineered for robust real-time PCR with earlier quantification cycle values (Ct), industry-leading sensitivity (increased limit of detection) and exceptional speed (rapid extension rates). The proprietary buffer system allows for highly efficient amplification of GC-rich and AT-rich sequences in addition to dramatic improvements in PCR sensitivity in low- copy assays and PCR conditions conducive to ultra-fast amplification. The Radiant™ Probe 1-Step Lo-ROX qPCR Kit utilizes a proprietary, Green non-inhibitory intercalating dye for robust, fluorescent detection.
New England Biolabs, Inc. LyoPrime Luna Probe One-Step RT-qPCR Mix with UDG - 120 rxns
The LyoPrime Luna Probe One-Step RT-qPCR Mix with UDG is offered in a lyophilized format allowing for ambient/room temperature shipping and storage prior to use. The mix contains all necessary components for one-step RT-qPCR except primers probe(s) and RNA sample. It can be reconstituted by simply adding water to a 2-4X concentration allowing for increased sample input. Performance in multiplexing applications has been optimized with sensitive linear detection achieved for up to 5 targets across a range of inputs.
TRIBIOSCIENCE INC 4-in-1 Aspergillus qPCR Kit, 100 Reactions
The 4-in-1 Aspergillus qPCR Kit is designed for identifying 4 Aspergillus species of A. flavus, A. fumigatus, A. niger, and A. terreus in a single PCR reaction using real-time quantitative polymerase chain reaction (qPCR) and probe fluorescence labels.
ACROBiosystems ResDetect™ Pichia Pastoris ResDNA Quantitation Kit, qPCR, 100 Tests
The resDetect™ Pichia pastoris resDNA Quantitation Kit is designed for the rapid and reliable quantitative detection of residual host cell DNA in biopharmaceuticals, such as antibodies and vaccines, using real-time quantitative PCR (qPCR). This kit provides high sensitivity with a lower limit of quantitation of 3 fg/μL and an LLOD of 1 fg/μL. Total assay procedures are typically completed in less than 4 hours.
- High sensitivity for optimal product safety: LLOD of 1 fg/μL.
- High specificity: No cross-reactivity with unrelated DNA.
- Validation: Conforms to ICH Q2(R2) analytical procedures.
- High-quality: Manufactured in an ISO 13485 standard facility.
- Wide dynamic range: Suitable for quantitative analysis of residual host cell DNA.
Alkali Scientific Radiant™ Probe Lo-ROX qPCR Kit, 100 Reactions, (1 x 1mL)
Alkali Scientific offers Radiant™ Probe Lo-ROX qPCR Kits, a high-performance portfolio of 2X ready-to-use real-time PCR reagents designed for best-in-class quantitative PCR. Radiant™ qPCR Kits are engineered for robust real-time PCR with earlier quantification cycle values (Ct), industry-leading sensitivity (increased limit of detection) and exceptional speed (rapid extension rates). The proprietary buffer system allows for highly efficient amplification of GC-rich and AT-rich sequences in addition to dramatic improvements in PCR sensitivity in low-copy assays and PCR conditions conducive to ultra-fast amplification. Furthermore, Radiant™ Probe Lo-ROX qPCR Kits are optimized for advanced multiplexing capability, delivering the same high efficiency, early Ct, and reaction speed even in complex multiplex assays.
LUNA NANOTECH INC Ngsprep Size Selection Magnetic Beads, 50 ML
PuroMAG™ NGSPrep Magnetic Beads, 50 mL
The PuroMAG™ NGSPrep Magnetic Beads are designed for fast and highly effective DNA clean-up after polymerase chain reaction (PCR, qPCR, ddPCR), microarrays, sequencing and other types of ezymatic reactions by removing unincorporated primers, dNTPs (deoxynucleotide triphosphates), salts, and other impurities. Combination of SPRI technology and paramagnetic feature of the beads allows for specific size selection of DNA fragments during the clean-up procedure with high recovery of the DNA product. This is crucial application in library preparation for various sequencing techniques, including next-generation sequencing (NGS).
Features
- Easy and fast clean-up of DNA PCR samples with efficient recovery of the product
- Highly efficient size selection of purified fragmented genome or PCR product for NGS library prep
- High throughput clean-up of 96/384 samples
- Can be used o
LUNA NANOTECH INC Maxi Silica Spin Columns, 50 Columns
PuroSPIN™ MAXI Spin Columns for DNA / RNA Extraction and Purification, 50 columns
Have you ever ran out of columns that come with your favorite plasmid extraction kit but still had a lot of left-over buffer reagents remaining? Instead of buying a new expensive kit you can use PuroSPIN™ columns with the left-over buffers as a cost-effective alternative. Simply use PuroSPIN™ columns while following the manufacturer-specific protocols.
Nucleic acid binding capacity: 2.3 mg of genomic DNA, 500 µg of plasmid DNA
Compatible as cost-effective replacement columns for Plasmid DNA purification kits from Qiagen, ThermoFisher, Bio-Rad, Promega and other manufacturers
PuroSPIN™ MAXI silica spin columns are designed for fast, simple and efficient extraction of Plasmid DNA from bacterial cultures
Biotium Fast Probe Master Mix (5000 rxn)
Fast Probe Master Mix is formulated for conducting real-time PCR using oligo-based probes such as TaqMan or MGB probes. The master mix contains buffer, dNTPs, MgCl2 and our superior hotstart enzyme Cheetah™ Taq. Cheetah™ Taq is a chemically modified hotstart Taq which can be fully activated in 2 minutes, thus permitting fast PCR. We offer two formats of probe master mix, without ROX and with ROX.For sensitive, affordable qPCR reactions we now recommend our new and improved Forget-Me-Not™ Universal Probe PCR Master Mix.TaqMan is a registered trademark of Roche Diagnostics.
Biochain Institute Inc dATP, dCTP, dGTP, dTTP, 4x 100 ul (100 mM)/PK
Polymerase Chain Reaction (PCR) has become one of the most important technologies in the scientific community since its introduction in 1985. BioChain provides the most common used qPCR and PCR enzyme and reagents, such as qPCR SuperMix, Taq polymerase, dNTP, primers, at the most competitive price. This product line offers our customers of genomic DNA, RNA, and cDNA, a convenient and economic source for large quantity PCR enzymes and reagents.
Biochain Institute Inc dATP, dCTP, dGTP, dTTP, 4x400 ul (100 mM)/PK
BioChain's dNTP reagent combines the highest quality with the most competitive price on the market. The dNTPs are purified with preparative HPLC to achieve at least 99.5% purity.
LUNA NANOTECH INC 4-Positions Maxi Magnetic Rack For 50 ML Tubes
Convenient MAXI format magnetic racks for large scale separation of magnetic beads in 50 mL centrifuge tubes. These racks can be used with our PuroMAG MAXI kits as well as various magnetic beads offered by Luna Nanotech and other manufactureres.